초록접수 현황
| 17F-225 | 구연 발표 |
Effect of C-reactive Protein Deposition on Myocardial Area at Risk : Novel Insight from Global MicroRNA Profilling in Ischemia-reperfusion Injury Rat Model
Se Jin Oh¹, Eun Na Kim², ChongJai Kim², Ki-Bong Kim³, Han Seok Choe⁴, Yun-Yong Park⁴
¹Department of Thoracic and Cariovascular Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul, Republic of Korea., ²Department of Pathology, Asan Medical Center, Ulsan University College of Medicine, Seoul, Republic of Korea., ³Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea., ⁴Asan Institute for Life Science, Asan Medical Center, Ulsan University College of Medicine, Seoul, Republic of Korea
Purpose : We previously reported the active participation of the deposition of C-reactive protein (CRP) on area at risk in the progression of myocardial infarction following ischemia-reperfusion injury. In the present study, we aimed to characterize global changes in microRNA (miR) expression in myocardial area at risk by ischemia-reperfusion injury with CRP infusion in rat.
Methods : Myocardial ischemia-reperfusion injury model was produced by ligation of the left anterior descending coronary artery for 45 minutes followed by 45 minutes of reperfusion using Sprague-Dawley rats. Experimental protocol is composed of the sham group, the ischemia-reperfusion group (I/R only), and the ischemia-reperfusion with CRP injection group (I/R+CRP). For the I/R+CRP group, 100 mg bolus of high-purity human CRP was infused. Evans blue and 2,3,5-triphenyltetrazolium chloride staining enables the discrimination of non-ischemia area, area at risk, and infarct area. After microRNA was extracted in each area, global microRNA was profiled by the digital multiplexed NanoString nCounter microRNA expression assay and nSolver software analysis.
Results : Comparison of the microRNA data within the each group identified many microRNAs expressed differently between the sham versus I/R only group, and the sham versus I/R+CRP group. Expression of rno-miR-1 (Rattus norvegicus microRNA-1, p=0.009) and rno-miR-674-3p (p=0.047) in the I/R+CRP group were significantly lower than the I/R only group (Figure 1).
Conclusion : The miR-1 is known as the important regulator of heart adaptation and is dysregulated in human myocardial infarction. Our study strongly suggests that rno-miR-1 dysregulation may be a linking mechanism between CRP deposition on myocardial area at risk and aggravation of myocardial infarction.
Methods : Myocardial ischemia-reperfusion injury model was produced by ligation of the left anterior descending coronary artery for 45 minutes followed by 45 minutes of reperfusion using Sprague-Dawley rats. Experimental protocol is composed of the sham group, the ischemia-reperfusion group (I/R only), and the ischemia-reperfusion with CRP injection group (I/R+CRP). For the I/R+CRP group, 100 mg bolus of high-purity human CRP was infused. Evans blue and 2,3,5-triphenyltetrazolium chloride staining enables the discrimination of non-ischemia area, area at risk, and infarct area. After microRNA was extracted in each area, global microRNA was profiled by the digital multiplexed NanoString nCounter microRNA expression assay and nSolver software analysis.
Results : Comparison of the microRNA data within the each group identified many microRNAs expressed differently between the sham versus I/R only group, and the sham versus I/R+CRP group. Expression of rno-miR-1 (Rattus norvegicus microRNA-1, p=0.009) and rno-miR-674-3p (p=0.047) in the I/R+CRP group were significantly lower than the I/R only group (Figure 1).
Conclusion : The miR-1 is known as the important regulator of heart adaptation and is dysregulated in human myocardial infarction. Our study strongly suggests that rno-miR-1 dysregulation may be a linking mechanism between CRP deposition on myocardial area at risk and aggravation of myocardial infarction.
첨부파일 : Figure 1..pdf
책임저자: Se Jin Oh
Department of Thoracic and Cariovascular Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul, Republic of Korea
발표자: Se Jin Oh, E-mail : wpwnn@hanmail.net