초록접수 현황
| 17F-050 | 포스터 발표 |
Migration of Lung Cancer Cells in a 3D Environment with Simulated Microgravity
¹Chi Bum Ahn, ¹Dae Geun Han, ¹Jin Woo Lee, ²Chang Hu Choi, ²Chul Hyun Park, ²Kook Yang Park, ²Kuk Hui Son
¹Department of Molecular Medicine, Gachon University Gil Medical Center, Gachon University Graduate School of Medicine, Incheon, Republic of Korea., ²Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, Gachon University College of Medicine, Incheon, Republic of Korea
Purpose : metastasis of cancer cells is one of the most important factors at the cancer therapy. Therefore, many people have studied the cancer migration to understand cancer biology. Recently, it has been reported that a migration speed is modulated by a variation of an external environment. Especially, a behavior of cancer cells in microgravity condition has gained attention as a tool for development of a new cancer therapy. We estimated the migration of two types of lung cancer cell lines (A549, H1703) using bioreactor with microgravity.
Methods : We estimated the migration of two types of lung cancer cell lines (A549, H1703) using bioreactor with microgravity. And cancer cell migration was measured by a scratch test based wound healing. In addition, mRNA expressions were measured by q-PCR.
Results : When we measured the cell proliferation (24hrs and 48hrs) with/ without microgravity, a proliferation rate of A549 with microgravity was faster than that of control group. At the scratch test of A549 based on the wound healing model, wound widths in microgravity were reduced quickly. Although H1703 in microgravity environment showed an enhanced decrease of wound width than that of control group, migration speed of H1703 was slower than that of A549. Gene expressions of each condition at the 24hrs and 48hrs showed the similar trends with wound healing results.
Conclusion : A Migration of lung cancer was influenced by the external environment and the microgravity accelerated the cancer migration. In addition, there were variations by types of lung cancers. Our results will be a potential at cancer migration and metastasis studies
Methods : We estimated the migration of two types of lung cancer cell lines (A549, H1703) using bioreactor with microgravity. And cancer cell migration was measured by a scratch test based wound healing. In addition, mRNA expressions were measured by q-PCR.
Results : When we measured the cell proliferation (24hrs and 48hrs) with/ without microgravity, a proliferation rate of A549 with microgravity was faster than that of control group. At the scratch test of A549 based on the wound healing model, wound widths in microgravity were reduced quickly. Although H1703 in microgravity environment showed an enhanced decrease of wound width than that of control group, migration speed of H1703 was slower than that of A549. Gene expressions of each condition at the 24hrs and 48hrs showed the similar trends with wound healing results.
Conclusion : A Migration of lung cancer was influenced by the external environment and the microgravity accelerated the cancer migration. In addition, there were variations by types of lung cancers. Our results will be a potential at cancer migration and metastasis studies
책임저자: Kuk Hui Son
Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, Gachon University College of Medicine, Incheon, Republic of Korea
발표자: Kuk Hui Son, E-mail : dr632@gilhospital.com