초록접수 현황
| 17F-066 | 구연 발표 |
Rosuvastatin Attenuates Degenerative Calcification of Bioprosthetic Heart Valve Through the Reduction of Interleukin-6 and Bone Morphogenic Protein-2 Expression
Seung Hyun Lee, Byung Chul Chang, Young-Nam Youn, Hyun Chel Joo, Kyung-Jong Yoo, Sak Lee
Department of Thoracic and Cardiovascular Surgery, Severance Cardiovascular Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
Purpose : There are pathophysiologic similarities between calcification and atherosclerosis because both are the product of an active inflammatory process. The aim of this study was to examine the effects of statin on degenerative calcification in commercially available bioprosthetic valve tissue.
Methods : Twenty Sprague-Dawley rats were fed a high fat diet to induce hypercholesterolemia. Then, they were randomly divided into 2 groups according to statin intake (Control group, n=10: high fat diet only; Statin group, n=10: high fat diet with statin treatment). Serum lipid levels were measured just before the experiment and after 4 and 12 weeks. Tissue valve cusps were surgically implanted in rat dorsal subcutis at 4 weeks. After implantation, rosuvastatin was administered daily to statin group. Cusps were explanted at 12 weeks and calcium levels were determined by atomic absorption spectroscopy. Western blotting, histological and immunohistochemical analysis were conducted to identify the anti-calcification mechanism of the statin.
Results : Mean calcium level in control group was significantly higher than statin group in all kinds of tissue valves (Carpentier-Edwards: 2.67 ± 0.26 mg/g vs. 1.31 ± 0.40 mg/g; HancockTM: 2.70 ± 0.57 mg/g vs. 1.53 ± 0.34 mg/g; Mitroflow: 2.39 ± 0.71 mg/g vs. 1.26 ± 0.38 mg/g; TrifectaTM: 2.54 ± 0.42 mg/g vs 1.63 ± 0.72 mg/g). Inflammatory cell infiltration and interleukin-6 and bone morphogenic protein-2 expressions were significantly reduced in statin group.
Conclusion : Statin treatment significantly attenuated degenerative calcification of bioprosthetic valve tissue through decrease the expression levels of Il-6 and BMP-2. Statin might be helpful to prolong the durability of bioprosthetics.
Methods : Twenty Sprague-Dawley rats were fed a high fat diet to induce hypercholesterolemia. Then, they were randomly divided into 2 groups according to statin intake (Control group, n=10: high fat diet only; Statin group, n=10: high fat diet with statin treatment). Serum lipid levels were measured just before the experiment and after 4 and 12 weeks. Tissue valve cusps were surgically implanted in rat dorsal subcutis at 4 weeks. After implantation, rosuvastatin was administered daily to statin group. Cusps were explanted at 12 weeks and calcium levels were determined by atomic absorption spectroscopy. Western blotting, histological and immunohistochemical analysis were conducted to identify the anti-calcification mechanism of the statin.
Results : Mean calcium level in control group was significantly higher than statin group in all kinds of tissue valves (Carpentier-Edwards: 2.67 ± 0.26 mg/g vs. 1.31 ± 0.40 mg/g; HancockTM: 2.70 ± 0.57 mg/g vs. 1.53 ± 0.34 mg/g; Mitroflow: 2.39 ± 0.71 mg/g vs. 1.26 ± 0.38 mg/g; TrifectaTM: 2.54 ± 0.42 mg/g vs 1.63 ± 0.72 mg/g). Inflammatory cell infiltration and interleukin-6 and bone morphogenic protein-2 expressions were significantly reduced in statin group.
Conclusion : Statin treatment significantly attenuated degenerative calcification of bioprosthetic valve tissue through decrease the expression levels of Il-6 and BMP-2. Statin might be helpful to prolong the durability of bioprosthetics.
책임저자: Sak Lee
Department of Thoracic and Cardiovascular Surgery, Severance Cardiovascular Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
발표자: Seung Hyun Lee, E-mail : henry75@yuhs.ac