초록접수 현황
| 17F-086 | 구연 미채택시 포럼 발표 |
The Effect on Monocyte with Early Application of Adsorption Filter in Swine Trauma Model
Do Wan Kim, Gwan-Sic Kim, Kyo Seon Lee, Yo Chun Jung, Sang Ki Oh, Sang Yun Song, KooK Joo Na, Bong Suk Oh, Byoung Hee Ahn, In Seok Jeong
Department of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital, Chonnam National University College of Medicine, Gwangju, Republic of Korea
Purpose : Monocytes have an important role to modulate the pro- and anti-inflammation in early phase of trauma. Hemoperfusion with adsorption filter has been expected to have an immune-modulatory function in septic patients. In this study, we investigated the effect of early application of adsorption filter on monocyte in trauma animal model.
Methods : Severe inflammation was experimentally induced by blunt thoracic injury and hemorrhagic shock in 20 pigs. We managed the control group with only fluid resuscitation and vasopressor, and the others were additionally treated with Polymyxin–B adsorption hemofilter (adsorption group). In filter group, Hemoperfusion was started at 1 hour after induction of injury, and maintained for 6 hours. Blood samples were taken at baseline, 1 and 7 hours after injury. We applied the flow cytometric analyses (CD14, CD16) to evaluate monocyte subtypes, and measured the mitochondrial function of monocyte with oxygen consumption rate (OCR).
Results : Mean body weight was 34.3±27.1 kg. By comparison of 2 groups after cessation of treatment, the ratio of total monocyte was statistically decreased in the adsorption group (p=0.03), and the ratio of classical type in monocyte subsets which has been known as the pro-inflammatory mediator, also statistically decreased in the adsorption group (p=0.003). In mitochondrial function test, the OCR was generally improved in adsorption group, whereas decreased in control group (p=0.001).
Conclusion : We confirmed the application of hemoperfusion with adsorption filter potentially improves the mitochondrial function in monocyte, and this type of modality should have an anti-inflammatory effect in early phase of trauma. However further investigation will be needed.
Methods : Severe inflammation was experimentally induced by blunt thoracic injury and hemorrhagic shock in 20 pigs. We managed the control group with only fluid resuscitation and vasopressor, and the others were additionally treated with Polymyxin–B adsorption hemofilter (adsorption group). In filter group, Hemoperfusion was started at 1 hour after induction of injury, and maintained for 6 hours. Blood samples were taken at baseline, 1 and 7 hours after injury. We applied the flow cytometric analyses (CD14, CD16) to evaluate monocyte subtypes, and measured the mitochondrial function of monocyte with oxygen consumption rate (OCR).
Results : Mean body weight was 34.3±27.1 kg. By comparison of 2 groups after cessation of treatment, the ratio of total monocyte was statistically decreased in the adsorption group (p=0.03), and the ratio of classical type in monocyte subsets which has been known as the pro-inflammatory mediator, also statistically decreased in the adsorption group (p=0.003). In mitochondrial function test, the OCR was generally improved in adsorption group, whereas decreased in control group (p=0.001).
Conclusion : We confirmed the application of hemoperfusion with adsorption filter potentially improves the mitochondrial function in monocyte, and this type of modality should have an anti-inflammatory effect in early phase of trauma. However further investigation will be needed.
첨부파일 : HP figure.pptx
책임저자: In Seok Jeong
Department of Thoracic and Cardiovascular Surgery, Chonnam National University Hospital, Chonnam National University College of Medicine, Gwangju, Republic of Korea
발표자: In Seok Jeong, E-mail : isjeong1201@gmail.com